Human inducible nitric oxide synthase,iNOS ELISA KIT

Human inducible nitric oxide synthase,iNOS ELISA KIT

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Mfr.No.

CSB-E08148h

Sensitivity

0.225 IU/mL

Detection Range

0.9 IU/mL-60 IU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

nitric oxide synthase 2, inducible, HEP-NOS, INOS, NOS, NOS2A, NOS, type II, nitric oxide synthase 2A, nitric oxide synthase 2A (inducible, hepatocytes), nitric oxide synthase, macrophage

Recovery

The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-105
1:2	Average %	96
	Range %	92-102
1:4	Average %	103
	Range %	97-107
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.375	2.294	2.335	2.160
100	1.903	1.802	1.853	1.678
50	1.355	1.376	1.366	1.191
25	0.903	0.914	0.909	0.734
12.5	0.650	0.641	0.646	0.471
6.25	0.423	0.410	0.417	0.242
3.12	0.317	0.312	0.315	0.140
0	0.176	0.173	0.175	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35228

Applications	ELISA
Research Area	NOS2

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.