Human Immunoglobulin M,IgM ELISA Kit

Mfr.No.

CSB-E07976h

Alternative Names

Immunoglobulin M,IgM

Reactivity

Human

Detection Range

4.68 ng/mL-300 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Urine

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	102
	Range %	98-107
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	95-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	2.651	2.536	2.594	2.440
300	2.049	1.928	1.989	1.835
150	1.376	1.315	1.346	1.192
75	0.892	0.952	0.922	0.768
37.5	0.568	0.529	0.549	0.395
18.75	0.374	0.364	0.369	0.215
9.4	0.237	0.241	0.239	0.085
0	0.150	0.157	0.154	?

Species

Homo sapiens

Sensitivity

1.17 ng/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin M,IgM

Applications

ELISA

Research Area

IgM

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of Lactococcus lactis and Enterococcus faecium on growth performance, digestive enzymes and immune response of grouper Epinephelus coioides Y.-Z. SUN et al,Aquaculture Nutrition,2012 June; 18(3): 281-289 Effects of heat-treatment on plasma rich in growth factors-derived autologous eye drop Anitua E et al,Exp Eye Res,2013 Dec 15.pii: S0014-4835(13)00349-7.

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SKU	Supplier No.	Size	Your price
BHE10502826	CSB-E07976h	96T	$305	Add to Cart
BHE10502826	CSB-E07976h	5x96T	$1464	Add to Cart
BHE10502826	CSB-E07976h	10x96T	$2897.50	Add to Cart

Human Immunoglobulin M,IgM ELISA Kit

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