Human Immunoglobulin M,IgM ELISA Kit

Mfr.No.

CSB-E07976h

Sensitivity

1.17 ng/mL

Detection Range

4.68 ng/mL-300 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Immunoglobulin M,IgM

Recovery

The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	102
	Range %	98-107
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	95-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of Lactococcus lactis and Enterococcus faecium on growth performance, digestive enzymes and immune response of grouper Epinephelus coioides Y.-Z. SUN et al,Aquaculture Nutrition,2012 June; 18(3): 281-289 Effects of heat-treatment on plasma rich in growth factors-derived autologous eye drop Anitua E et al,Exp Eye Res,2013 Dec 15.pii: S0014-4835(13)00349-7.

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