Human Immunoglobulin J Chain

Mfr.No.

E3420Hu

Alternative Names

Immunoglobulin J chain;JCHAIN;Joining chain of multimeric IgA and IgM

Reactivity

Human

Detection Range

30-9000ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

18099

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

16.25ng/mL

Background

Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.

Gene ID	3512
UniProt ID	P01591
Antigen	JCHAIN

Research Area

Immunology

Protocol

These standard curves of E3420Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/mL	2.223	2.299	2.266
	2.374
2400ng/mL	1.332	1.345	1.312
	1.358
1200ng/mL	0.624	0.606	0.573
	0.588
600ng/mL	0.33	0.361	0.328
	0.391
300ng/mL	0.126	0.132	0.099
	0.138
0ng/mL	0.038	0.033	0
	0.028

Precision

We measured random samples of E3420Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2151	98.99	4.6
Intra-Assay	2	18	3129.6	111.72	3.6
Intra-Assay	3	18	755.7	36.4	4.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104947	E3420Hu	48T	$320		Add to Cart
BHE12104947	E3420Hu	96T	$458		Add to Cart

Human Immunoglobulin J Chain - IGJ ELISA Kit

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