Human Immunoglobulin G(IgG)ELISA Kit

Human Immunoglobulin G(IgG)ELISA Kit

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Mfr.No.

CSB-E07979h

Alternative Names

Immunoglobulin G(IgG)

Reactivity

Human

Detection Range

0.59 ug/mL-150 ug/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma, Urine, Cell culture supernatants

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	93
	Range %	85-107
1:2000	Average %	95
	Range %	87-104
1:4000	Average %	89
	Range %	84-100
1:8000	Average %	90
	Range %	81-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	Corrected
40	2.345	2.412	2.379	2.279
10	2.176	2.210	2.193	2.093
2.5	1.682	1.657	1.670	1.570
0.625	1.237	1.253	1.245	1.145
0.156	0.783	0.802	0.793	0.693
0.039	0.429	0.436	0.433	0.333
0.01	0.272	0.281	0.277	0.177
0	0.101	0.098	0.100	?

Species

Homo sapiens

Sensitivity

0.487 ug/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin G(IgG)

Applications

ELISA

Research Area

IgG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of heat-treatment on plasma rich in growth factors-derived autologous eye drop Anitua E et al,Exp Eye Res,2013 Dec 15.pii: S0014-4835(13)00349-7. Immunomodulatory constituents of human breast milk and immunity from bronchiolitis Chunyu Li.et al,Italian Journal of Pediatrics,Jan 14

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.