Human immunoglobulin binding protein,BIP ELISA KIT

Human immunoglobulin binding protein,BIP ELISA KIT

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Mfr.No.

CSB-E12119h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), BIP, FLJ26106, GRP78, MIF2, Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD), heat shock 70kD protein 5 (glucose-regulated protein, 78kD), heat shock 70kDa protein 5

Recovery

The recovery of human TMEM95 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM95 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	100
	Range %	95-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.235	2.378	2.307	2.115
500	1.648	1.652	1.650	1.458
250	1.091	1.123	1.107	0.915
125	0.712	0.738	0.725	0.533
62.5	0.488	0.496	0.492	0.300
31.2	0.375	0.401	0.388	0.196
15.6	0.267	0.283	0.275	0.083
0	0.191	0.193	0.192	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11021

Applications	ELISA
Research Area	HSPA5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10502820	CSB-E12119h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.