Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®

Mfr.No.

EK0777

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.012	0.107	0.200	0.323	0.694	1.149	1.521	2.026

Description

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6R in cell culture supeatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin 6 receptor ;Interleukin 6 receptor, isoform CRA_a ;IL6R ;hCG_1810911 ;

Molecular Weight

51548 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-D358

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-6R alpha

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or Citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	54	271	821	59	259	773
Standard deviation	2.86	17.07	41.87	3.77	17.35	44.06
CV (%)	5.3%	6.7%	5.7%	6.4%	6.7%	5.7%

Background

IL6R alpha (IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alteatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	54	48	54	48	51	3	5.8%
Sample 2	271	254	262	234	255	13.66	5.3%
Sample 3	821	928	965	877	897	54.21	6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3570
Gene Name	IL6R
UniProt ID	A0N0L5
Protein Name	Interleukin-6 receptor subunit alpha

Applications	ELISA
Research Area	Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Interleukins,Lymphoid,Myeloid,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000615	EK0777	96 wells/kit	$499		Add to Cart

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®

Rate it now..!!