Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IL-4 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
97
EDTA plasma(n=5)
90-100
96
heparin plasma(n=5)
92-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-4 ELISA Kit allows for the in vitro quantitative determination of IL-4 concentrations in serum, plasma, tissue homogenates and other biological fluids.