Human IL-33 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0929

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.029	0.057	0.101	0.189	0.335	0.632	1.152	2.098

Description

Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL33 in cell culture supeatants, serum, plasma (heparin, EDTA) and cell lysates.

Alternative Names

C9orf26; DVS27; DVS27 related protein; IL-1F11; IL-33; IL1F11; IL33; IL33_HUMAN; Interleukin-1 family member 11; Interleukin-33; NF-HEV; NFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2;

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S112-T270

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-33

Subcellular Localization

Secreted, Nucleus, Chromosome, secretory vesicle

Sample Type

Cell culture supeatants, Serum, Plasma, Cell lysates

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	30	260	484	28	282	511
Standard deviation	1.74	16.38	37.75	1.76	22.27	45.99
CV (%)	5.8%	6.3%	7.8%	6.3%	7.9%	9%

Protein Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in tu activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.

Background

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	90865
Gene Name	IL33
UniProt ID	O95760
Tissue Specificity	Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cytokines,Hypertrophy,Immunology,Innate Immunity,Interleukins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000101	EZ0929	5 plates/kit	$500		Add to Cart

Human IL-33 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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