Human IL-31/Interleukin-31 ELISA Kit PicoKine®

Mfr.No.

EK0979

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.004	0.142	0.197	0.316	0.586	0.984	1.446	2.096

Description

Human IL-31 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL31 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-31;IL-31;IL31;

Molecular Weight

18205 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-T164

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-31

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	134	804	1907	140	740	1811
Standard deviation	8.04	44.22	122.04	10.5	52.54	130.39
CV (%)	6%	5.5%	6.4%	7.5%	7.1%	7.2%

Protein Function

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .

Background

Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	134	122	129	116	125	6.83	5.4%
Sample 2	804	765	827	741	784	33.38	4.2%
Sample 3	1907	1624	1623	1803	1739	121.45	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	386653
Gene Name	IL31
UniProt ID	Q6EBC2
Protein Name	Interleukin-31
Tissue Specificity	Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. .

Applications	ELISA
Research Area	Cytokines,Immunology,Innate Immunity,Interleukins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000742	EK0979	96 wells/kit	$499		Add to Cart

Human IL-31/Interleukin-31 ELISA Kit PicoKine®

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