Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IL-27 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-27 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-96
90
EDTA plasma(n=5)
89-98
93
heparin plasma(n=5)
85-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-27 ELISA Kit allows for the in vitro quantitative determination of IL-27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IL-27 (Interleukin-27 subunit alpha) ELISA Kit