Human IL-10 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0416

Detection Range

7.8 pg/ml - 500 pg/ml (cell culture supeates) 3.4 pg/ml - 250 pg/ml (human serum, plasma)

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.070	0.151	0.187	0.295	0.538	0.858	1.622	2.281

Description

Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL10 in cell culture supeatants, serum and plasma ( heparin, EDTA, citrate).

Alternative Names

Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;IL10;

Molecular Weight

20517 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-10

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Protein Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Background

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	3586
Gene Name	IL10
UniProt ID	P22301
Protein Name	Interleukin-10
Tissue Specificity	Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Sequence	Belongs to the IL-10 family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000112	EZ0416	5 plates/kit	$500		Add to Cart

Human IL-10 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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