Human IL-1 Receptor Like 1(IL1RL1)ELISA Kit

Human IL-1 Receptor Like 1(IL1RL1)ELISA Kit

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Mfr.No.

CSB-E13789h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

interleukin 1 receptor-like 1, DER4, FIT-1, MGC32623, ST2, ST2L, ST2V, T1, growth stimulation-expressed, homolog of mouse growth stimulation-expressed, interleukin 1 receptor-related protein

Recovery

The recovery of human FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-105
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-99
1:2	Average %	89
	Range %	84-96
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.989	2.036	2.013	1.811
500	1.621	1.641	1.631	1.429
250	1.116	1.132	1.124	0.922
125	0.682	0.698	0.690	0.488
62.5	0.498	0.506	0.502	0.300
31.2	0.375	0.389	0.382	0.180
15.6	0.286	0.299	0.293	0.091
0	0.203	0.201	0.202	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q01638
Tissue Specificity	Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C

Applications	ELISA
Research Area	IL1RL1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502816	CSB-E13789h	96T	$790	Add to Cart
BHE10502816	CSB-E13789h	5x96T	$3792	Add to Cart
BHE10502816	CSB-E13789h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.