IGHV3-9 ELISA Kit, Immunoglobulin heavy variable 3-9 ELISA Kit, Ig heavy chain V-III region DOB ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
31.2-2000pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IGHV3-9 and the recovery rates were calculated by comparing the measured value to the expected amount of IGHV3-9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
93
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
85-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGHV3-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-99%
91-102%
89-102%
EDTA plasma(n=5)
90-100%
85-92%
85-101%
heparin plasma(n=5)
86-97%
88-97%
90-95%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P01782
Applications
ELISA
Applications Notes
IGHV3-9 ELISA Kit allows for the in vitro quantitative determination of IGHV3-9 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IGHV3-9 (Immunoglobulin heavy variable 3-9) ELISA Kit