Matrices listed below were spiked with certain level of IgG4 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
95
EDTA plasma(n=5)
86-105
96
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IgG4 ELISA Kit allows for the in vitro quantitative determination of IgG4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IgG4(Immunoglobulin G4) ELISA Kit