Matrices listed below were spiked with certain level of IgG3 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
93
EDTA plasma(n=5)
86-102
95
heparin plasma(n=5)
85-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IgG3 ELISA Kit allows for the in vitro quantitative determination of IgG3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IgG3(Immunoglobulin G3) ELISA Kit