Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine®

Mfr.No.

EK0991

Sensitivity

<20 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.001	0.023	0.035	0.070	0.161	0.345	0.812	1.836

Description

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGFBP7 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Insulin-like growth factor-binding protein 7;IBP-7;IGF-binding protein 7;IGFBP-7;IGFBP-rP1;MAC25 protein;PGI2-stimulating factor;Prostacyclin-stimulating factor;Tumor-derived adhesion factor;TAF;IGFBP7;MAC25, PSF;

Molecular Weight

29130 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-L282

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IGFBP7

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1629	4265	18882	1617	4427	20605
Standard deviation	114.03	319.87	1340.62	118.04	358.58	1669
CV (%)	7%	7.5%	7.1%	7.3%	8.1%	8.1%

Protein Function

Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. .

Background

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1629	1498	1507	1608	1560	58.55	3.7%
Sample 2	4265	3682	4312	3781	4010	281.18	7%
Sample 3	18882	20935	19883	21797	20374	1096.22	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3490
Gene Name	IGFBP7
UniProt ID	Q16270
Protein Name	Insulin-like growth factor-binding protein 7
Sequence	Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Applications	ELISA
Research Area	Cancer,Growth Factors/Hormones,Oncoproteins/Suppressors,Signal Transduction,Tumor Suppressors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000753	EK0991	96 wells/kit	$450		Add to Cart

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine®

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