Human IGF2R/IGF-II R ELISA Kit PicoKine®

Mfr.No.

EK1326

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.023	0.222	0.406	0.673	1.136	1.538	1.824	2.072

Description

Human IGF2R/IGF-II R ELISA Kit PicoKine® (96 Tests). Quantitate Human IGF2R in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cation-independent mannose-6-phosphate receptor;CI Man-6-P receptor;CI-MPR;M6PR;300 kDa mannose 6-phosphate receptor;MPR 300;Insulin-like growth factor 2 receptor;Insulin-like growth factor II receptor;IGF-II receptor;M6P/IGF2 receptor;M6P/IGF2R;CD222;IGF2R;MPRI;

Molecular Weight

274375 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S1510-F2108

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IGF2R

Subcellular Localization

Lysosome membrane ; Single-pass type I membrane protein . Colocalized with DPP4 in intealized cytoplasmic vesicles adjacent to the cell surface.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	249	1351	4462	248	1314	4363
Standard deviation	9.96	77	197.32	13.64	94.42	253.05
CV (%)	4%	5.7%	4.4%	5.5%	7.3%	5.8%

Protein Function

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. .

Background

Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the mateal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the mateal allele.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	249	245	232	224	237	10.01	4.2%
Sample 2	1351	1344	1406	1445	1386	41.43	2.9%
Sample 3	4462	4544	5035	4402	4610	250.07	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3482
Gene Name	IGF2R
UniProt ID	P11717
Protein Name	Cation-independent mannose-6-phosphate receptor
Sequence	Belongs to the MRL1/IGF2R family.

Applications	ELISA
Research Area	Golgi Proteins,Immunology,Innate Immunity,Macrophage/Inflammation,Organelles,Protein Trafficking,Signal Transduction,Subcellular Markers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000964	EK1326	96 wells/kit	$499		Add to Cart

Human IGF2R/IGF-II R ELISA Kit PicoKine®

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