Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL011443HU

Reactivity

Human

Detection Range

0.039 ng/ml

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Duration

1-5h

Recovery

The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-105
EDTA plasma (n=4)	87	83-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-99
1:200	Average %	93
	Range %	88-97
1:400	Average %	85
	Range %	80-89
1:800	Average %	90
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.156 ng/ml -10 ng/ml

Limit Of Detection

450 nm

UniProt ID	P06312
Antigen	immunoglobulin kappa variable 4-1

Applications

ELISA

Research Area

IGKV4-1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10502814	CSB-EL011443HU	96T	$695	Add to Cart
BHE10502814	CSB-EL011443HU	5x96T	$3336	Add to Cart
BHE10502814	CSB-EL011443HU	10x96T	$6602.50	Add to Cart