Human iduronate sulfatase,IDS ELISA Kit

Human iduronate sulfatase,IDS ELISA Kit

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Mfr.No.

CSB-E09471h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

iduronate 2-sulfatase, MPS2, SIDS, alpha-L-iduronate sulfate sulfatase, iduronate 2-sulfatase 14 kDa chain, iduronate 2-sulfatase 42 kDa chain, iduronate-2-sulfatase, idursulfase

Recovery

The recovery of human DAG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-97
EDTA plasma (n=4)	88	81-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DAG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	98
	Range %	93-103
1:4	Average %	96
	Range %	91-102
1:8	Average %	95
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.301	2.159	2.230	2.098
4000	1.934	1.868	1.901	1.769
2000	1.562	1.534	1.548	1.416
1000	1.234	1.202	1.218	1.086
500	0.825	0.845	0.835	0.703
250	0.543	0.551	0.547	0.415
125	0.322	0.310	0.316	0.184
0	0.133	0.131	0.132

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P22304

Applications	ELISA
Research Area	IDS

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502804	CSB-E09471h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.