Human IDE(Insulin-degrading enzyme) ELISA Kit

Mfr.No.

EH1988

Alternative Names

IDE ELISA Kit, Insulin-degrading enzyme ELISA Kit, Abeta-degrading protease ELISA Kit, Insulin protease ELISA Kit, Insulinase ELISA Kit, Insulysin ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

62.5-4000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of IDE and the recovery rates were calculated by comparing the measured value to the expected amount of IDE in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	92-100	95
EDTA plasma(n=5)	90-105	97
heparin plasma(n=5)	87-104	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-105%	85-100%	92-101%
EDTA plasma(n=5)	83-98%	86-98%	82-97%
heparin plasma(n=5)	81-96%	83-94%	84-92%

Sensitivity

< 37.5pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

IDE/Insulin-degrading enzyme/Abeta-degrading protease/Insulin protease/Insulinase/Insulysin

Notes

For Research Use Only

UniProt ID	P14735
Antigen	IDE

Applications	ELISA
Applications Notes	IDE ELISA Kit allows for the in vitro quantitative determination of IDE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800446	EH1988	96T	$400		Add to Cart

Human IDE(Insulin-degrading enzyme) ELISA Kit

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