Human ICOS ligand(ICOSLG) ELISA kit

Human ICOS ligand(ICOSLG) ELISA kit

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Mfr.No.

CSB-EL010958HU

Sensitivity

23.4 pg/mL

Detection Range

93.75 pg/mL-6000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

inducible T-cell co-stimulator ligand, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, KIAA0653, LICOS, B7-like protein, transmembrane protein B7-H2 ICOS ligand

Recovery

The recovery of mouse LTF/LF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	103	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LTF/LF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-106
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-107
1:8	Average %	88
	Range %	82-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
200	0.137	0.138	0.138
100	0.225	0.231	0.228
50	0.393	0.382	0.388
25	0.668	0.678	0.673
12.5	1.141	1.136	1.139
6.25	1.671	1.698	1.685
3.12	1.915	1.854	1.885
0	2.121	2.264	2.193

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O75144

Applications	ELISA
Research Area	ICOSLG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502803	CSB-EL010958HU	96T	$695	Add to Cart
BHE10502803	CSB-EL010958HU	5x96T	$3336	Add to Cart
BHE10502803	CSB-EL010958HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.