Human ICOS(Inducible T-Cell Co Stimulator) ELISA Kit

Mfr.No.

ELK3134

Alternative Names

CD278; AILIM; Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant ICOS and the recovery rates were calculated by comparing the measured value to the expected amount of ICOS in samples.

Matrix	Recovery range	Average
serum(n=5)	82-98%	90%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	82-94%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ICOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	93-106%	82-95%	85-96%	90-98%
EDTA plasma(n=5)	82-93%	88-95%	96-102%	89-103%
Heparin plasma(n=5)	89-96%	90-99%	95-106%	87-101%

Typical Data

<table> <tbody><tr> <th>Concentration (pg/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>1000.00</td> <td>2.126</td> <td>2.041</td> </tr> <tr> <td>500.00</td> <td>1.629</td> <td>1.544</td> </tr> <tr> <td>250.00</td> <td>1.035</td> <td>0.950</td> </tr> <tr> <td>125.00</td> <td>0.828</td> <td>0.743</td> </tr> <tr> <td>62.50</td> <td>0.496</td> <td>0.411</td> </tr> <tr> <td>31.25</td> <td>0.327</td> <td>0.242</td> </tr> <tr> <td>15.63</td> <td>0.268</td> <td>0.183</td> </tr> <tr> <td>0.00</td> <td>0.085</td> <td>0.000</td> </tr> </tbody></table>

Sensitivity

5.8 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	Q9Y6W8
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICOS in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Signal transduction;Tumor immunity;Infection immunity;Immune molecule;
Protocol	<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3134-1.pdf" target="_blank">Datasheet</a>
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203161	ELK3134	48T	$320		Add to Cart
BHE15203161	ELK3134	96T	$458		Add to Cart

Human ICOS(Inducible T-Cell Co Stimulator) ELISA Kit

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