Human hypoxia-inducible factor 2(HIF-2) ELISA kit

Mfr.No.

CSB-E12113h

Alternative Names

endothelial PAS domain protein 1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, HIF-1alpha-like factor, basic-helix-loop-helix-PAS protein MOP2, hypoxia-inducible factor 2 alpha, member of PAS protein 2

Reactivity

Human

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human EDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	86
	Range %	81-92
1:4	Average %	94
	Range %	87-98
1:8	Average %	86
	Range %	80-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	1.832	1.988	1.910	1.802
150	1.214	1.192	1.203	1.095
75	0.760	0.721	0.741	0.633
37.5	0.442	0.451	0.447	0.339
18.75	0.287	0.276	0.282	0.174
9.375	0.212	0.217	0.215	0.107
4.688	0.155	0.160	0.158	0.050
0	0.107	0.108	0.108	?

Species

Homo sapiens

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q99814
Antigen	endothelial PAS domain protein 1

Applications

ELISA

Research Area

EPAS1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502802	CSB-E12113h	96T	$790	Add to Cart
BHE10502802	CSB-E12113h	5x96T	$3792	Add to Cart
BHE10502802	CSB-E12113h	10x96T	$7505	Add to Cart

Human hypoxia-inducible factor 2(HIF-2) ELISA kit

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