Human hypoxia-inducible factor 1alpha(HIF-1alpha) ELISA kit

Human hypoxia-inducible factor 1alpha(HIF-1alpha) ELISA kit

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Mfr.No.

CSB-E12112h

Alternative Names

hypoxia-inducible factor 1alpha(HIF-1alpha), HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, ARNT interacting protein, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, alpha subunit, hypoxia-inducible factor 1, alpha, hypoxia-inducible factor 1alpha(HIF-1alpha), hypoxia-inducible factor 1 alpha(HIF-1 alpha)

Reactivity

Human

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	95	88-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	82-105
1:2	Average %	92
	Range %	85-100
1:4	Average %	98
	Range %	92-107
1:8	Average %	90
	Range %	83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.337	2.404	2.371	2.222
250	1.692	1.659	1.676	1.527
125	1.106	1.138	1.122	0.973
62.5	0.675	0.662	0.669	0.520
31.2	0.436	0.428	0.432	0.283
15.6	0.257	0.273	0.265	0.116
7.8	0.206	0.215	0.211	0.062
0	0.153	0.145	0.149	?

Species

Homo sapiens

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q16665
Antigen	hypoxia-inducible factor 1α(HIF-1α)

Applications

ELISA

Research Area

HIF-1alpha

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502801	CSB-E12112h	96T	$790	Add to Cart
BHE10502801	CSB-E12112h	5x96T	$3792	Add to Cart
BHE10502801	CSB-E12112h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.