Human hydroxyproline,Hyp ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08837h

Sensitivity

19.5 ng/mL

Detection Range

78 ng/mL-5000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

hydroxyproline,Hyp

Recovery

The recovery of pig D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	89-107
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-99
1:2	Average %	92
	Range %	85-100
1:4	Average %	90
	Range %	84-99
1:8	Average %	96
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Hyp

Storage	Store at 2-8°C. Please refer to protocol.

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