Human Hyaluronidase Ph-20 - SPAM1 ELISA Kit

Mfr.No.

E5438Hu

Sensitivity

4.95ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Hyal-PH20;Hyaluronidase PH-20;Hyaluronoglucosaminidase PH-20;SPAM 1;SPAM1;Sperm adhesion molecule 1;Sperm surface protein PH-20

Molecular Weight

57848

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5438Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	255	12.29	4.8
Intra-Assay	2	18	839.6	25.87	3.1
Intra-Assay	3	18	508	19.35	3.8

Background

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Protocol

These standard curves of E5438Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.018	1.936	1.898
	1.854
600ng/L	1.095	1.073	1.034
	1.05
300ng/L	0.419	0.517	0.479
	0.616
150ng/L	0.175	0.192	0.153
	0.209
75ng/L	0.122	0.135	0.096
	0.147
0ng/L	0.052	0.039	0
	0.025

Gene ID	6677
UniProt ID	P38567

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106800	E5438Hu	48T	$320		Add to Cart
BHE12106800	E5438Hu	96T	$458		Add to Cart

Human Hyaluronidase Ph-20 - SPAM1 ELISA Kit

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