Human Hyaluronidase-1 - HYAL1 ELISA Kit

Mfr.No.

E5439Hu

Description

This sandwich kit is for the accurate quantitative detection of human Hyaluronidase-1 (also known as HYAL1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

HYAL1;HYAL-1;Hyaluronidase-1;Hyaluronoglucosaminidase-1;LUCA1;LuCa-1;Lung carcinoma protein 1;MPS9;NAT6;

Reactivity

Human

Detection Range

0.5-180ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

48368

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.34ng/ml

Background

May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.

Gene ID	3373
UniProt ID	Q12794
Antigen	HYAL1

Research Area

Others

Protocol

These standard curves of E5439Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
96ng/ml	2.653	2.588	2.533
	2.524
48ng/ml	1.409	1.434	1.379
	1.459
24ng/ml	0.897	0.916	0.861
	0.936
12ng/ml	0.524	0.492	0.437
	0.461
6ng/ml	0.221	0.227	0.172
	0.234
0ng/ml	0.049	0.055	0
	0.062

Precision

We measured random samples of E5439Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	25.13	1.38	5.48
Intra-Assay	2	18	43.68	1.31	3
Intra-Assay	3	18	19.39	1.4	7.22

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106801	E5439Hu	48T	$320		Add to Cart
BHE12106801	E5439Hu	96T	$458		Add to Cart

Human Hyaluronidase-1 - HYAL1 ELISA Kit

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