Human Hyaluronic acid,HA ELISA Kit

Human Hyaluronic acid,HA ELISA Kit

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Mfr.No.

CSB-E04805h

Alternative Names

Hyaluronic acid,HA

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Plasma, Cerebrospinal fluid, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human Dsg-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-96
EDTA plasma (n=4)	87	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Dsg-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-102
1:2	Average %	94
	Range %	86-98
1:4	Average %	93
	Range %	89-97
1:8	Average %	99
	Range %	95-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.308	2.512	2.410	2.285
750	1.342	1.445	1.394	1.269
375	0.779	0.757	0.768	0.643
187.5	0.443	0.457	0.450	0.325
94	0.312	0.303	0.308	0.183
47	0.231	0.234	0.233	0.108
23.5	0.198	0.192	0.195	0.070
0	0.124	0.125	0.125

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

Antigen	Hyaluronic acid,HA

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Intense pulsed light induces synthesis of dermal extracellular proteins in vitro Cuerda-Galindo E. et al,Lasers Med Sci,2015 Jul 19. Increased fibroblast proliferation and activity after applying intense pulsed light 800-1200 nm. Cuerda-Galindo E.et al,Ann Anat.,198:66-72 Increased fibroblast proliferation and activity after applying intense pulsed light 800-1200 nm Cuerda-Galindo E.et al,Ann Anat.,2015 198:66-72 Cartilage oligomeric matrix protein and hyaluronic acid are sensitive serum biomarkers for early cartilage lesions in the knee joint Jiao Q. et al,Biomarkers,2015 Dec 3:1-6. The Relationship of the Degree of Hepatic Fibrosis with Hyaluronic Acid, Type 4 Collagen, and Procollagen Type 3 N-Terminal Peptide Levels in Patients with Chronic Viral Hepatitis kader IRAk. et al,Viral Hepatitis Journal,Viral Hepatitis Journal21(1): 8-12 METHOD OF ENHANCING HYALURONIC ACID SECRETION USING PROBIOTIC STRAIN Y Lin. et al,U.S. Patent,2015 Skin Photo-Protective and Anti-ageing Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation Bonnie P. Hegarty-Diaz.et al,Journal of Diseases and Medicinal Plants,May 8 Impact of Consciousness Energy Healing Treatment on Herbomineral Formulation in Different Skin Health Parameters Deborah Lea Smith.et al,American Journal of Biomedical and Life Sciences,May 3 Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation Dennille Mellesia Smith.et al,Journal of Food and Nutrition Sciences,May 9 Biofield Energy Healing Based Herbomineral Formulation: An Emerging Frontier in Cosmetic Medicine Dezi Ann Koster.et al,American Journal of Biomedical and Life Sciences,May 9 An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection EileenMary Meagher.et al,American Journal of Laboratory Medicine,May 2 Skin Rejuvenating Effect of Consciousness Energy Healing Treatment Based Herbomineral Formulation James Jeffery Peoples.et al,American Journal of Plant Biology,May 9 Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters Janice Patricia Kinney.et al,American Journal of Life Sciences,May 9 The Study of Biofield Energy Treatment Based Herbomineral Formulation in Skin Health and Function Johanne Dodon.et al,American Journal of BioScience,May 9 Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it’s implication for wound healing Mahendra Kumar Trivedi.et al,Journal of Food Science and Technology,27 February 2017 Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect Mahendra Trivedi.et al,American Journal of Life Sciences,May 8 Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation Shirley Theresa Holmlund.et al,European Journal of Preventive Medicine,May 3 The Use of Consciousness Energy Healing Based Herbomineral Formulation for Skin Anti-Aging Strategies Vaibhav Rajan Parulkar1.et al,Journal of Food and Nutrition Sciences,May 9 Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula William Dean Plikerd.et al,American Journal of Health Research,May 9 Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health William Dean Plikerd.et al,American Journal of Health Research,May 8 Clinical significance of decoy receptor 3 upregulation in patients with hepatitis B and liver fibrosis Xiaoli Lou.et al,Oncology Letters,May 21 Evaluation of antioxidant, immunostimulatory and antifatigue properties of Dashmularishta using in vitro and in vivo assays Gupta, Arun.et al,indian journal of experimental biology,August

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.