Human Hyaluronan-binding protein 2(HABP2) ELISA kit

Human Hyaluronan-binding protein 2(HABP2) ELISA kit

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Mfr.No.

CSB-EL010113HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

hyaluronan binding protein 2, FSAP, HABP, HGFAL, PHBP, factor VII activating protein, hepatocyte growth factor activator-like protein, hyaluronic acid binding protein 2, plasma hyaluronan binding protein

Recovery

The recovery of human MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-105
EDTA plasma (n=4)	96	87-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	85
	Range %	80-90
1:10	Average %	92
	Range %	87-96
1:20	Average %	85
	Range %	82-92
1:40	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.126	2.052	2.089	1.977
4000	1.359	1.276	1.318	1.206
2000	0.784	0.734	0.759	0.647
1000	0.459	0.439	0.449	0.337
500	0.348	0.324	0.336	0.224
250	0.256	0.238	0.247	0.135
125	0.181	0.171	0.176	0.064
0	0.115	0.109	0.112	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q14520
Tissue Specificity	Ubiquitously expressed.

Applications	ELISA
Research Area	HABP2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502785	CSB-EL010113HU	96T	$695	Add to Cart
BHE10502785	CSB-EL010113HU	5x96T	$3336	Add to Cart
BHE10502785	CSB-EL010113HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.