Matrices listed below were spiked with certain level of HSPA13 and the recovery rates were calculated by comparing the measured value to the expected amount of HSPA13 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
97-102
100
EDTA plasma(n=5)
88-102
94
heparin plasma(n=5)
90-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSPA13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-103%
92-101%
86-105%
EDTA plasma(n=5)
85-99%
94-98%
83-93%
heparin plasma(n=5)
89-100%
83-88%
85-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P48723
Applications
ELISA
Applications Notes
HSPA13 ELISA Kit allows for the in vitro quantitative determination of HSPA13 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HSPA13 (Heat shock 70 kDa protein 13)ELISA Kit