Matrices listed below were spiked with certain level of HSP72 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP72 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-104
100
EDTA plasma(n=5)
87-104
97
heparin plasma(n=5)
85-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP72 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HSP72 ELISA Kit allows for the in vitro quantitative determination of HSP72 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HSP72(Heat Shock Protein 72) ELISA Kit