Human HSP27/HSPB1/Heat Shock Protein 27 ELISA Kit PicoKine®

Mfr.No.

EK0881

Sensitivity

<5pg/ml

Detection Range

62.5pg/ml-4000pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.099	0.119	0.173	0.253	0.384	0.697	1.127	1.796

Description

Human HSP27 / Heat Shock Protein 27 ELISA Kit PicoKine® (96 Tests). Quantitate Human HSPB1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28;

Molecular Weight

22783 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T2-K205

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HSP27

Subcellular Localization

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	82	492	1757	81	468	1729
Standard deviation	3.44	26.07	110.69	4.53	33.69	121.03
CV (%)	4.2%	5.3%	7.2%%	5.6%	7.2%	7%

Protein Function

Involved in stress resistance and actin organization.

Background

Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.

Reproducibility

Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	82	88	78	91	84	5.06	6%
Sample 2	492	526	485	501	501	15.5	3%
Sample 3	1757	1541	1607	1549	1613	86.67	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3315
Gene Name	HSPB1
UniProt ID	P04792
Protein Name	Heat shock protein beta-1
Tissue Specificity	Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .
Sequence	Belongs to the small heat shock protein (HSP20) family.

Applications	ELISA
Research Area	Contractility,Heat Shock Proteins,Protein Trafficking,Signal Transduction,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000674	EK0881	96 wells/kit	$380		Add to Cart

Human HSP27/HSPB1/Heat Shock Protein 27 ELISA Kit PicoKine®

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