Matrices listed below were spiked with certain level of HSF1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSF1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
93
EDTA plasma(n=5)
93-104
99
heparin plasma(n=5)
87-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HSF1/HSTF1/heat shock factor protein 1/heat shock transcription factor 1HSTF1HSF 1
Notes
For Research Use Only
UniProt ID
Q00613
Antigen
HSF1
Applications
ELISA
Applications Notes
HSF1 ELISA Kit allows for the in vitro quantitative determination of HSF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HSF1(Heat shock factor protein 1) ELISA Kit