Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit

Mfr.No.

ELK8920

Alternative Names

Amyloid Beta Peptide 1-42;

Reactivity

Human

Detection Range

4.69-300 pg/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant hs Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of hs Ab1-42 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-102%	95%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	90-105%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	78-96%	78-93%	82-90%
EDTA plasma(n=5)	95-102%	93-101%	81-89%	85-99%
Heparin plasma(n=5)	89-97%	96-103%	97-105%	89-97%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
300.00	2.204	2.119
150.00	1.628	1.543
75.00	1.144	1.059
37.50	0.882	0.797
18.75	0.533	0.448
9.38	0.385	0.300
4.69	0.168	0.083
0.00	0.085	0.000

Sensitivity

1.35 pg/mL

Limit Of Detection

300 pg/mL

Antigen	hsAb1-42
Usage Notes	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human hs Ab1-42 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Neuro science;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200145	ELK8920	48T	$320		Add to Cart
BHE15200145	ELK8920	96T	$458		Add to Cart

Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit

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