Human hs Ab1-40(high sensitivity Amyloid Beta Peptide 1-40) ELISA Kit

Mfr.No.

ELK8921

Sensitivity

1.42 pg/mL

Detection Range

4.69-300 pg/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Competitive Inhibition

Alternative Names

Amyloid Beta Peptide 1-40;

Recovery

Matrices listed below were spiked with certain level of recombinant hs Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of hs Ab1-40 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-103%	96%
EDTA plasma(n=5)	83-97%	90%
Heparin plasma(n=5)	86-99%	97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	97-106%	79-93%	82-90%
EDTA plasma(n=5)	95-102%	89-96%	87-98%	85-93%
Heparin plasma(n=5)	89-103%	85-92%	95-103%	87-93%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
300.00	2.401	2.328
150.00	1.563	1.490
75.00	1.128	1.055
37.50	0.897	0.824
18.75	0.576	0.503
9.38	0.313	0.240
4.69	0.196	0.123
0.00	0.073	0.000

Limit Of Detection

300 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human hs Ab1-40 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Infection immunity;Neuro science;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200144	ELK8921	48T	$320		Add to Cart
BHE15200144	ELK8921	96T	$458		Add to Cart

Human hs Ab1-40(high sensitivity Amyloid Beta Peptide 1-40) ELISA Kit

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