Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of HRH1 and the recovery rates were calculated by comparing the measured value to the expected amount of HRH1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
98
EDTA plasma(n=5)
86-98
93
heparin plasma(n=5)
86-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HRH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-103%
88-105%
88-105%
EDTA plasma(n=5)
85-96%
85-100%
89-98%
heparin plasma(n=5)
87-100%
83-100%
80-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P35367
Applications
ELISA
Applications Notes
HRH1 ELISA kit allows for the in vitro quantitative determination of HRH1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HRH1 (Histamine H1 receptor) ELISA kit