Human HLA class II histocompatibility antigen gamma chain(CD74) ELISA kit

Human HLA class II histocompatibility antigen gamma chain(CD74) ELISA kit

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Mfr.No.

CSB-EL004956HU

Alternative Names

CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAG, HLADG, Ia-GAMMA, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma

Reactivity

Human

Detection Range

0.9 ng/mL-60 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-100
EDTA plasma (n=4)	99	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	96
	Range %	90-102
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.356	2.378	2.367	2.252
4000	2.121	2.161	2.141	2.026
2000	1.757	1.798	1.778	1.663
1000	1.343	1.391	1.367	1.252
500	0.851	0.899	0.875	0.760
250	0.502	0.539	0.521	0.406
125	0.272	0.291	0.282	0.167
0	0.114	0.116	0.115	?

Species

Homo sapiens

Sensitivity

0.225 ng/mL

Limit Of Detection

450 nm

UniProt ID	P04233
Antigen	CD74 molecule, major histocompatibility complex, class II invariant chain

Applications

ELISA

Research Area

CD74

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502764	CSB-EL004956HU	96T	$695	Add to Cart
BHE10502764	CSB-EL004956HU	5x96T	$3336	Add to Cart
BHE10502764	CSB-EL004956HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.