Human HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA) ELISA kit

Human HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA) ELISA kit

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Mfr.No.

CSB-EL010497HU

Alternative Names

HLA class II histocompatibility antigen, DR alpha chain, DASS-397D15.1, HLA-DRA1, HLA class II histocompatibility antigen, DR alpha chain, MHC cell surface glycoprotein, histocompatibility antigen HLA-DR alpha major histocompatibility complex, class II, DR alpha(HLA-DRA)

Reactivity

Human

Detection Range

18.75 pg/mL-1200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-95
1:200	Average %	94
	Range %	90-98
1:400	Average %	99
	Range %	95-104
1:800	Average %	102
	Range %	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
20	2.496	2.537	2.517	2.390
10	2.181	2.192	2.187	2.060
5	1.795	1.741	1.768	1.641
2.5	1.353	1.313	1.333	1.206
1.25	0.878	0.898	0.888	0.761
0.625	0.369	0.366	0.368	0.241
0.312	0.242	0.259	0.251	0.124
0	0.123	0.130	0.127	?

Species

Homo sapiens

Sensitivity

4.68 pg/mL

Limit Of Detection

450 nm

UniProt ID	P01903
Antigen	HLA class II histocompatibility antigen, DR alpha chain

Applications

ELISA

Research Area

HLA-DRA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10502763	CSB-EL010497HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.