Human Histone H4 - HIST1H4A ELISA Kit

Mfr.No.

E5420Hu

Sensitivity

10.33ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

H4C1;Histone H4

Molecular Weight

11367

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5420Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	341.2	13.78	4
Intra-Assay	2	18	1248.6	30.3	2.4
Intra-Assay	3	18	1117.8	58.25	5.2

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protocol

These standard curves of E5420Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.306	2.29	2.245
	2.274
1200ng/L	1.205	1.279	1.234
	1.354
600ng/L	0.714	0.643	0.597
	0.571
300ng/L	0.292	0.337	0.291
	0.381
150ng/L	0.205	0.186	0.141
	0.168
0ng/L	0.045	0.045	0
	0.046

Gene ID	8359
UniProt ID	P62805

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106782	E5420Hu	48T	$320		Add to Cart
BHE12106782	E5420Hu	96T	$458		Add to Cart

Human Histone H4 - HIST1H4A ELISA Kit

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