Human Histone H1.3 - HIST1H1D ELISA Kit

Mfr.No.

E5415Hu

Sensitivity

7.36ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

H1.3;H1D;H1F3;H1s-2;HIST1H1D;Histone H1.3;Histone H1c;Histone H1s-2

Molecular Weight

22350

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5415Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1019.05	26.68	2.62
Intra-Assay	2	18	585.3	27.7	4.73
Intra-Assay	3	18	290.49	13.04	4.49

Background

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Protocol

These standard curves of E5415Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.158	2.122	2.089
	2.087
800ng/L	1.117	1.09	1.056
	1.063
400ng/L	0.535	0.564	0.53
	0.592
200ng/L	0.24	0.241	0.207
	0.242
100ng/L	0.111	0.105	0.071
	0.099
0ng/L	0.041	0.034	0
	0.026

Gene ID	3007
UniProt ID	P16402

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106777	E5415Hu	48T	$320		Add to Cart
BHE12106777	E5415Hu	96T	$458		Add to Cart

Human Histone H1.3 - HIST1H1D ELISA Kit

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