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Home / ELISA Kit

Human Histamine N-methyltransferase - HNMT ELISA Kit

SKU : BHE12106767

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Sample Request

Mfr.No.

E5405Hu

Sensitivity

7.86ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Histamine N-methyltransferase;HMT;HNMT

Molecular Weight

33295

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5405Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	608.2	33.18	5.5
Intra-Assay	2	18	766.9	21.26	2.8
Intra-Assay	3	18	192.7	9.09	4.7

Background

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.

Protocol

These standard curves of E5405Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.288	2.216	2.141
	2.143
800ng/L	1.301	1.381	1.307
	1.461
400ng/L	0.865	0.78	0.706
	0.696
200ng/L	0.412	0.423	0.349
	0.435
100ng/L	0.3	0.285	0.21
	0.269
0ng/L	0.077	0.074	0
	0.072

Gene ID	3176
UniProt ID	P50135
Antigen	HNMT

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106767	E5405Hu	48T	$320		Add to Cart
BHE12106767	E5405Hu	96T	$458		Add to Cart

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