Human Histamine H2 Rceptor

Mfr.No.

E6826Hu

Sensitivity

0.015ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Gastric receptor I;H2R;HH2R;Histamine H2 receptor;HRH 2;HRH2

Molecular Weight

40098

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6826Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.2	0.1	4.8
Intra-Assay	2	18	3.5	0.11	3
Intra-Assay	3	18	1.4	0.06	4.1

Background

The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).

Protocol

These standard curves of E6826Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.298	2.128	2.091
	1.958
4ng/ml	1.573	1.538	1.501
	1.503
2ng/ml	0.677	0.722	0.685
	0.768
1ng/ml	0.43	0.403	0.366
	0.375
0.5ng/ml	0.175	0.171	0.134
	0.167
0ng/ml	0.038	0.037	0
	0.036

Gene ID	3274
UniProt ID	P25021

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115575	E6826Hu	48T	$320		Add to Cart
BHE12115575	E6826Hu	96T	$458		Add to Cart

Human Histamine H2 Rceptor - HRH2 ELISA Kit

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