Human Histamine H1 Receptor

Mfr.No.

E6825Hu

Sensitivity

0.053ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

H1R;HH1R;Histamine H1 receptor;HRH 1;HRH1

Molecular Weight

55784

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6825Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.4	0.22	5
Intra-Assay	2	18	11.4	0.4	3.5
Intra-Assay	3	18	7.7	0.41	5.3

Background

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

Protocol

These standard curves of E6825Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.29	2.172	2.146
	2.055
12ng/ml	1.475	1.425	1.399
	1.376
6ng/ml	0.641	0.673	0.647
	0.706
3ng/ml	0.436	0.416	0.39
	0.396
1.5ng/ml	0.14	0.176	0.149
	0.211
0ng/ml	0.031	0.026	0
	0.022

Gene ID	3269
UniProt ID	P35367

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115574	E6825Hu	48T	$320		Add to Cart
BHE12115574	E6825Hu	96T	$458		Add to Cart

Human Histamine H1 Receptor - HRH1 ELISA Kit

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