Human high sensitivity C-C motif chemokine 2 (CCL2) ELISA kit

Human high sensitivity C-C motif chemokine 2 (CCL2) ELISA kit

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Mfr.No.

CSB-EQ004783HU

Alternative Names

C-C motif chemokine 2, HC11_x000D_ Monocyte chemoattractant protein 1_x000D_ Monocyte chemotactic and activating factor_x000D_ Short name=MCAF_x000D_ Monocyte chemotactic protein 1_x000D_ Short name=MCP-1_x000D_ Monocyte secretory protein JE_x000D_ Small-inducible cytokine A2_x000D_ MCP1, SCYA2_x000D_, C-C motif chemokine 2, HC11_x000D_ Monocyte chemoattractant protein 1_x000D_ Monocyte chemotactic and activating factor_x000D_ Short name=MCAF_x000D_ Monocyte chemotactic protein 1_x000D_ Short name=MCP-1_x000D_ Monocyte secretory protein JE_x000D_ Small-inducible cytokine A2_x000D_ MCP1, SCYA2_x000D_

Reactivity

Human

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse SEMA3D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	98	92-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	99
	Range %	96-102
1:8	Average %	96
	Range %	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.433	2.332	2.383	2.224
750	2.049	1.928	1.989	1.830
375	1.339	1.318	1.329	1.170
187.5	0.727	0.741	0.734	0.575
94	0.485	0.496	0.491	0.332
47	0.280	0.291	0.286	0.127
23.5	0.221	0.225	0.223	0.064
0	0.159	0.158	0.159	?

Species

Homo sapiens

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	P13500
Antigen	high sensitivity C-C motif chemokine 2 (CCL2)

Applications

ELISA

Research Area

CCL2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502738	CSB-EQ004783HU	96T	$595	Add to Cart
BHE10502738	CSB-EQ004783HU	5x96T	$2856	Add to Cart
BHE10502738	CSB-EQ004783HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.