Human High mobility group protein B1,HMGB-1 ELISA Kit

Mfr.No.

CSB-E08223h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

high-mobility group box 1, DKFZp686A04236, HMG1, HMG3, SBP-1, Amphoterin, OTTHUMP00000018196, OTTHUMP00000190860, OTTHUMP00000200117, Sulfoglucuronyl carbohydrate binding protein, high mobility group box 1, high mobility group prot

Recovery

The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-103
EDTA plasma (n=4)	102	96-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	102
	Range %	96-107
1:4	Average %	94
	Range %	87-98
1:8	Average %	86
	Range %	81-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
3000	2.066	2.154	2.110	1.992
1500	1.496	1.589	1.543	1.425
750	1.007	1.043	1.025	0.907
375	0.634	0.647	0.641	0.523
187.5	0.375	0.382	0.379	0.261
93.75	0.267	0.273	0.270	0.152
46.88	0.186	0.189	0.188	0.070
0	0.117	0.118	0.118	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P09429

Applications	ELISA
Research Area	HMGB1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502735	CSB-E08223h	96T	$856	Add to Cart
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BHE10502735	CSB-E08223h	10x96T	$8132	Add to Cart

Human High mobility group protein B1,HMGB-1 ELISA Kit

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