Human High affinity immunoglobulin gamma Fc receptor I(FCGR1A) ELISA kit

Human High affinity immunoglobulin gamma Fc receptor I(FCGR1A) ELISA kit

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Mfr.No.

CSB-EL008537HU

Sensitivity

0.2 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Fc fragment of IgG, high affinity Ia, receptor (CD64), CD64, CD64A, FCRI, FLJ18345, IGFR1, Fc fragment of IgG, high affinity Ia, receptor for (CD64), Fc gamma receptor, Fc-gamma RI, Fc-gamma receptor I A1, IgG Fc receptor I

Recovery

The recovery of human MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-101
1:2	Average %	99
	Range %	92-108
1:4	Average %	94
	Range %	90-98
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.508	2.607	2.558	2.415
250	1.765	1.864	1.815	1.672
125	1.187	1.206	1.197	1.054
62.5	0.791	0.801	0.796	0.653
31.2	0.447	0.438	0.443	0.300
15.6	0.302	0.311	0.307	0.164
7.8	0.238	0.232	0.235	0.092
0	0.142	0.143	0.143	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P12314

Applications	ELISA
Research Area	FCGR1A

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502731	CSB-EL008537HU	96T	$695	Add to Cart
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BHE10502731	CSB-EL008537HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.