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Home / ELISA Kit

Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit

SKU : BHE13702820

Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated.The deduced 190-amino acid human CTR1 protein is similar to yeast CTR1 and Arabidopsis COPT1, a copper transporter also isolated by functional complementation of yeast ctr1. All 3 predicted proteins have 3 transmembrane domains and an N terminus that is rich in methionine and serine residues; the N terminus of human CTR1 is also abundant in histidines. Northern blot analysis detected 2 major CTR1 transcripts of approximately 2 kb and 5.5 kb and a less abundant transcript of about 8.5 kb in all human organs and tissues examined.

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Mfr.No.	KTE60604
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC31A1.
Alternative Names	SLC31A1; COPT1; CTR1; MGC75487; hCTR1; copper transport 1 homolog; copper transporter 1
Detection Method	Colorimetric
Kit Components	Human High affinity copper uptake protein 1 microplate Human High affinity copper uptake protein 1 standard Human High affinity copper uptake protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC31A1. No significant cross-reactivity or interference between Human SLC31A1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated.The deduced 190-amino acid human CTR1 protein is similar to yeast CTR1 and Arabidopsis COPT1, a copper transporter also isolated by functional complementation of yeast ctr1. All 3 predicted proteins have 3 transmembrane domains and an N terminus that is rich in methionine and serine residues; the N terminus of human CTR1 is also abundant in histidines. Northern blot analysis detected 2 major CTR1 transcripts of approximately 2 kb and 5.5 kb and a less abundant transcript of about 8.5 kb in all human organs and tissues examined.
Notes	This Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC31A1 in samples. An antibody specific for SLC31A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC31A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC31A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC31A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1317

Applications	ELISA
Applications Notes	This Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC31A1 in samples. An antibody specific for SLC31A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC31A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC31A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC31A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702820	KTE60604	48 T	$339		Add to Cart
BHE13702820	KTE60604	96 T	$589		Add to Cart
BHE13702820	KTE60604	96 Tx5	$2629		Add to Cart
BHE13702820	KTE60604	96 Tx50	$24569		Add to Cart

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