Human Hexokinase 3 - HK3 ELISA Kit

Mfr.No.

E0732Hu

Sensitivity

0.021ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Hexokinase type III;Hexokinase-3;Hexokinase-C;HK 3;HK III;HK3

Molecular Weight

99025

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0732Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.4	0.48	7.5
Intra-Assay	2	18	2.3	0.14	6.1
Intra-Assay	3	18	1.5	0.09	5.9

Background

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed: 8717435). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed: 8717435).

Protocol

These standard curves of E0732Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.088	2.107	2.056
	2.127
6ng/mL	1.729	1.767	1.716
	1.806
3ng/mL	1.095	1.153	1.101
	1.211
1.5ng/mL	0.633	0.686	0.634
	0.738
0.75ng/mL	0.359	0.392	0.34
	0.425
0ng/mL	0.046	0.052	0
	0.058

Gene ID	3101
UniProt ID	P52790

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102411	E0732Hu	96T	$458		Add to Cart
BHE12102411	E0732Hu	48T	$320		Add to Cart

Human Hexokinase 3 - HK3 ELISA Kit

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