Human Heterogeneous Nuclear Ribonucleoprotein A1

Mfr.No.

E5396Hu

Sensitivity

0.14ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Helix-destabilizing protein;Heterogeneous nuclear ribonucleoprotein A1;Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed;hnRNP A1;hnRNP core protein A1;HNRNPA 1;HNRNPA1;Single-strand RNA-binding protein

Molecular Weight

38747

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5396Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	30.8	0.62	2
Intra-Assay	2	18	20.9	0.96	4.6
Intra-Assay	3	18	9.6	0.53	5.6

Background

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection (PubMed: 17371836). May bind to specific miRNA hairpins (PubMed: 28431233). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed: 31$498.00|$360.00791). (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

Protocol

These standard curves of E5396Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.277	2.278	2.233
	2.278
24ng/mL	1.213	1.233	1.188
	1.253
12ng/mL	0.576	0.662	0.617
	0.748
6ng/mL	0.383	0.406	0.361
	0.43
3ng/mL	0.166	0.186	0.141
	0.206
0ng/mL	0.049	0.045	0
	0.04

Gene ID	3178
UniProt ID	P09651

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106758	E5396Hu	48T	$320		Add to Cart
BHE12106758	E5396Hu	96T	$458		Add to Cart

Human Heterogeneous Nuclear Ribonucleoprotein A1 - HNRNP A1 ELISA Kit

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