Human hepcidin(Hepc) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13062h

Alternative Names

hepcidin antimicrobial peptide, HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide, putative liver tumor regressor

Reactivity

Human

Detection Range

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Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

UniProt ID	P81172
Antigen	hepcidin antimicrobial peptide

Applications

ELISA

Research Area

HAMP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502706	CSB-E13062h	10x96T	$7505	Add to Cart